Some Virulence Factors Genes and Phylogenic Groups of Uropathogenic Escherichia Coli (UPEC) Isolated from Karbala Patients | ||
| Kerbala Journal of Medicine | ||
| Article 1, Volume 9, Issue 1, June 2016, Pages 2376-2385 PDF (0 K) | ||
| Authors | ||
| Ahmed K. A AL-Ganimi; Jawad K.T AL-Khafaji | ||
| Abstract | ||
| background: Uropathogenic Escherichia coli (UPEC) is most important causative agent of urinary tract infection (UTI). This disease is still big health problem among human population in spite of scientific progress. Aims: aims of this study were to investigate the isolates of UPEC in patients with UTI, characterize the virulence factors and phylogenic groups among clinical isolates. Materials and Methods: A total of 150 urine specimens were collected from patients with UTI after establishing the diagnosis via investigation and clinical diagnosis in Al-Hussein Hospital, Gynecology and Obstetrics Hospital, and Children Hospital in Karbala province during the period from November 2013 to April 2014. Thirteen stool samples from apparently healthy individuals as control group were included. All E. coli isolates were identified by standard methods. Molecular diagnosis and characterization for virulence and phylogenic genes were detected by Multiplex PCR technique in medical researches laboratory of medical college at Karbala University. Results and Discussion: This study showed that 56/150 patients (37.3%) had UTI due to UPEC. The isolated UPEC were examined for the presence of the adhesion genes (Papc, Afa and Sfa) and the phylogeny groups’ genes by Multiplex PCR assays. The primers encoding virulence genes were tested against to all the 56 UPEC and 13 stool isolates. It was found that 27(48.21%) urine isolates carried just one virulence factor, of them 16(28.57%) isolates carrying papc, 2(3.57%) isolates carrying afa and 9(16.07%) isolates carrying sfa. No isolates out of 56 had carried all the three genes. However, no virulence factor was found in 24 (42.85%) isolates. Pap gene was found mostly in 7 isolates among patients aged (1-10 years) and (21-30 years); while, the prevalence of sfa gene was found in 7 isolates (50%) among patients aged (31-40 years); however, two isolates have afa gene it was in one isolate in each age group (1-10 years) and (41-50 years). The percentage of phylogenic groups of UPEC isolates belonging to B2 followed by D, A and B1, were 39.28%, 33.92%, 16.07% and 10.71% respectively; while in control group the results were A (53.84%), B2 (23.07%), B1 (15.38%) and D (7.69%). Conclusions: Its̓ concluded that the genes of virulence factors of UPEC isolates in patients with UTI were higher than in healthy persons. Significantly, E. coli strains responsible for UTI were far more likely to be members of phylogenic groups B2 or D than A or B1. | ||
| Keywords | ||
| Virulence factor genes; Phylogenic groups; UPEC and UTI | ||
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