Effect of salinity on callus Induction from seedling of sweet pepper Capsicum annuum L. in vitro | ||
| JURNAL OF MADENAT ALELEM COLLEGE | ||
| Article 1, Volume 8, Issue 1, June 2016, Pages 39-47 | ||
| Authors | ||
| Jenan A. Al Ani; Zahra N. Al Hattab; Wajida K. Al Qaudhy; Ekhlas A. Al Kaaby; Ashwaq A; Ammar K. S | ||
| Abstract | ||
| The local sweet pepper cultivar was used in this study. Sterilized seeds were growing on different germination media to germinate and obtaining seedling. Full strength MS medium supplemented with1 mg/L GA3 and agar was superior as compare to medium containing 7 gm/l agar and distilled water and MS medium with agar ,the germination percentage reached ( 86.7%) as compare to ( 55% and 23.3%) for both last two medium respectively. Moreover The results showed that the leaves gave significant differences in the fresh and dry weight of the induced calli, they were 0.248 g and 0.034 g respectively compared with 0.198 g and 0.017 g respectively for the calli produced from the hypocotyls. The results showed that the salinity had negative effect on callus growth. The control treatment with 6 ECdS.m-1 gave the highest calli fresh and dry weight 198.69 mg and 21.97 mg compared with 132.70 mg and 11.36 mg for 10 ECdS.m-1 treatments. | ||
| Keywords | ||
| Salt stress; hypocotyls | ||
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