Detection of Tomato yellow leaf curl virus (TYLCV) and its strains on Tomato plants and host rang determine by Polymerase Chain Reaction | ||
| Kufa Journal for Agricultural Science | ||
| Article 1, Volume 6, Issue 4, December 2014, Pages 99-117 | ||
| Authors | ||
| Al- Jubouri A.A; Al- Fadhal F. A; Samaka H. M | ||
| Abstract | ||
| This study was conducted to identify , determine the host rang, detection of virus strains and find out the source of initial infection of Tomato yellow leaf curl virus (TYLCV) on tomato Solanum lycopersicom L. ,weeds and other economic crops planted closed to tomato fields in the desert region of Najaf province by polymerase chain reaction (PCR) technique. Samples of tomato leaves showed curling , rolling, stunting and yellowing symptoms were collected from the hybrids Aula,Aseel ,Shreen and Abu- alkaf . PCR results showed that tomato plants have two strains of TYLCV which were occupied area strain (Palestine ) TYLCV-IS and normal strain TYLCV-mld in Sheen hybrid, by amplifying two DNA bands with sizes of 634bp and 316bp respectively , where the other TYLCV -infected hybrids were given a 634bp DNA band , in addition PCR results showed that the economic plants used in this study Eggplant (Solanum melongen L.) , Melon ( Cucumis melo L.) , Cucumber ( Cucumis sativus L.) , Sesame (Sesamum indicum L.) and Cowpea (Vigna sinensis L.) and Weeds plants Small flowered mallow (Malva parviflora L.) , Wall goosefoot (Chenopodium murale L.),and Kinoa (Chenopodium quinoa Willd.) , gave positive results with TYLCV-IS strain by amplifying a 634bp – DNA band representing the occupied area strain. While Common sow thistle (Sonchus oleraceus L.) and Pop saltbush (Atriplex holocarpa F- muell.), gave negative result by PCR test. | ||
| Keywords | ||
| TYLCV virus; PCR technique; Host Range | ||
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