Application of RAPD Markers for Genetic Distance Assessing of Sunflower (Helianthus annuusL.) Lines | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 8, Issue 1, March 2016, Pages 109-119 | ||
| Authors | ||
| N. Abdul H. M. Al-Badeiri; M. A. Al-anbari; H. M. K. al-khafaji | ||
| Abstract | ||
| This study was carried out at the biology science department/College of Education-girls/kufa university Genetic Laboratories during 2015. The objective was to investigate genetic diversity among six sunflower lines. The random amplified polymorphic DNA (RAPD) based on PCR with 10 primers. Were used RAPD DNA markers were used to evaluate trends in genetic diversity among six of inbred line .All of the eleven RAPD primers produced 110 bands with average 11 bands for each primer. The number of amplified fragments produced per primer ranged from 8 for OPC-04 primer to 14 for OPA-17, primer with molecular size ranged from 123 to 1495 bp. The total number of polymorphic fragment and the percentage of polymorphism bands (50, 45.09 %) respectively. Maximum level of polymorphism was 63.64% 0bserved for the primer OPA-19 while Primer OPA-16 showed the lowest percentage of polymorphism reached 30% for OPA-19 and OPM-13 primers. Maximum level of primer efficiency for OPA-17 was 12.73% and were reached 14%. Based on the bivariate ( 1 - 0 ) data and genetic similarity with the use of UPGMA cluster method, the dendrogram separated the studied populations in to A and B. Cluster and secondary cluster analysis which compared between inbred lines in to dendrogram. Showed BK104 high genetic diversity was high genetic diversity between C and A . Genetic Distance that the highest estimate 0.74 was observed between line C , A . While, the lowest genetic Distance between inbred line I , E and Q , I (0.30, 0.32) respectively. Results indicated that RAPD markers were highly efficient in electing the genetic diversity among sunflower lines. | ||
| Keywords | ||
| RAPD; PCR; Homozygosity primers and Inbred lines | ||
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