Study of Fibrinolytic Enzyme (Streptokinase) Produced from Clinical Streptococcus pyogenes Isolates | ||
| Journal of University of Babylon | ||
| Article 1, Volume 24, Issue 2, February 2016, Pages 364-369 | ||
| Authors | ||
| Eman M . Jarallah; Ahmed Abbas A; Noor Saadallah N; Zahraa Abed Neama | ||
| Abstract | ||
| Thirty samples of bacterial isolates were collected from patients of Hilla Teaching Hospital during the period from 1st - 31 st / July – 2014 . These samples were isolated from upper respiratory tract (pharynx) of both sex patients . The swabs were cultured immediately on tryptic soya agar and incubated at 37 C for 24 hr . The isolates were identified according to cultural properties and biochemical testes . The results showed that these isolates were belonged to Streptococcus pyogenes . The ability of these isolates to produce streptokinase was studied . The results exhibited presence of two active SPA1 and SPA2 . The activity of the produced enzyme was studied by fibrin plate method . Furthermore the effect of pH at different values and different temperatures ( -5,5,25,35 and 55C) were examined . The results revealed that the optimum pH was 6 , while the optimum temperature was 35C . | ||
| Keywords | ||
| Streptokinase; Streptococcus pyogenes; Fibrinolytic Enzyme | ||
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