Image analysis method to evaluate biological control of Aspergillus flavus growth by Bacillus subtilis and Pseudomonas fluorescens | ||
basrah journal of science | ||
Article 1, Volume 32, Issue 2, December 2014, Pages 290-303 PDF (0 K) | ||
Authors | ||
Labeed A. Al-Saad; Sami A. Al-Jumaily; Adnan I. Al-Badran | ||
Abstract | ||
This study was aimed to evaluate the ability of some biocontrol agents to suppress the growth of the Aspergillus flavus biologically. Five isolates of Bacillus subtilis and two of Pseudomonas fluorescens were examined to evaluate their ability to inhibit fungal growth in vitro on solid media. Growth inhibition estimated according to growth area measurement which made by analysis of images that picked up by digital camera and analyzed by UTHSCSA ImageTool, v. 2.0, software that developed by the University of Texas Health Science Center, San Antonio. Results of growth inhibition percentages showed superiority of B. subtilis isolate BSS4 (99.47%) and P. fluorescens isolates PFDL (92.29%) & PFMst (86.19%) respectively with no significant differences among them followed by B. subtilis isolates BSS2 (74.47%) & BSW (72.67%) which differed significantly from BSS4. | ||
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