Effect of vitamin E plus Selenium against lead exposure on histopathology of kidney and liver in common carp Cyprinus carpio L | ||
| Al-Anbar Journal of Veterinary Sciences | ||
| Article 1, Volume 8, Issue 1, June 2015, Pages 24-32 | ||
| Authors | ||
| S. A. Mustafa; A. M. Alkhalili | ||
| Abstract | ||
| The present study was undertaken to investigate the effects of lead (Pb) either alone or with Vitamin E (VE) plus Selenium (Se) on histopathological changes in Cyprinus carpio. For this purpose, 120 common carp with an average weight 65.50±0.25 g, and total length 17.4±2.5 cm were randomly divided into 6 treatment groups in duplicate. Each of the six treatment groups was fed the formulated diets for 10 weeks at a daily rate of 3% body weight throughout the experiment as the following: Treatment 1 (T1) serve as control group; Treatment 2 (T2) were fed diet mixed with VE + Se (300 mg kg-1 d.w); Treatment 3 (T3) were fed diet mixed with Pb (20 mg kg-1 d.w; Treatment 4 (T4) were fed diet mixed with Pb (30 mg kg-1 d.w); Treatment 5 (T5) were fed diet mixed with Pb (20 mg kg-1 d.w) with VE+Se; Treatment 6 (T6) were fed diet mixed with Pb (30 mg kg-1 d.w) with VE+Se. After 10 weeks feeding trail 3 fish tank-1 (6 fish treatment-1) were dissected out liver and kidney were removed and fixed in 10% formaldehyde solution for histopathological studies. The liver of control group exhibited a quite normal architecture, while the fish exposed to Pb showed vacuolation and necrosis. These hepatic alterations were more evident in fish exposed to (30mg kg-1 dw). The kidney exhibited Bowman space dilatation, acute cellular degeneration and necrosis. The quantitative histopathology revealed abnormalities in kidney and liver in all dietary Pb treated groups alone and with VE+Se. The severity of lesion in both organs were significantly different (P<0.05) in Pb groups in comparison to Pb with VE+Se groups. These results indicated that histopathology could be used as a tool for assessing the risk of contaminants in the environment | ||
| Keywords | ||
| Cyprinus carpio; Lead; Selenium; vitamin E; Histopathology | ||
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