Effect of G1705A SNP in Growth Hormone Gene on the Productive and Physiological Performance in Broiler Chicken | ||
| Iraqi Journal of Biotechnology | ||
| Article 1, Volume 15, Issue 1, April 2016, Pages 33-45 | ||
| Authors | ||
| Dihya H. H. Al-Hassani; Bassam G. M. Al-khatib | ||
| Abstract | ||
| In this study two commercial broiler hybrids were used (Cobb500 and Hubbard-F15) to detect the G1705A SNP in the third intron of chicken GH gene and investigate its effect on the productive and physiological performance in broiler chicken. PCR- RFLP method was used to identify this SNP, three genotypes were found when using of Eco RV restriction enzyme wild genotype GG, heterozygous GA and homozygous AA. Highly significant difference (p<0.01) was found between the distribution of the different genotypes, the genotype GG had the highest percentage followed by GA then AA and allele G had the superiority over allele A in both broiler breed, no significant effect of the various genotypes on the productive traits in both broiler breeds of study, significant effect (p<0.05) of the various genotypes were found on the serum total protein and triglycerides concentrations of Cobb500 at 14 days of age and the genotypes GG and GA were gave highest mean followed by the genotype AA. | ||
| Keywords | ||
| GH Gene; PCR; RFLP; Eco RV; A SNP | ||
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