Production of Pure Isolates For Some Strains of Cultivated Mushroom Agaricus bisporus by Using Technology of Tissue Culture | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 16, Issue 1, March 2016, Pages 72-80 | ||
| Author | ||
| Mustafa Rashid Majeed Alkaisi | ||
| Abstract | ||
| The aim of this research is to test the efficiency of some strains of Agaricus bisporus, media and laboratory technologies in the production of pure isolates Through the application of technology of tissue culture. The strains which enter that’s X25, X20 and B62 with four kind of media have different carbon sources between complex to simple they PDA, Malt extract, Czapek-Agar%20 Sucrose, Czapek-Agar%20 Glucose. And it was the standard of efficiency for strains and media the percent of mycelium initiation successful from tissue of fruit body and speed of mycelium initiation For their confine of increase the damage percent which produce from fungal and bacterial infections death of the self-tissue, It has been applied additional procedure For the purpose of reduce the occurrence of infections through sterilization of the fruit body as a whole by a flame and the addition of anti-bacterial to the media. It has been found Superiority the X25 and X20 strains after 10 days of culture began to increase the success rate of tissue culture to 75% and 45.83% sequentially on B62 strain 33.33% and the superiority the two media Czapek-Agar% 20 Glucose and Czapek-Agar% 20 Sucrose to 46.67% on each Malt extract and PDA, which rated to 13.33% and 5% sequentially in the indicator of mycelium initiation speed. | ||
| Keywords | ||
| pure isolates; cultivated mushroom; Tissue culture | ||
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