Production of Biological De-Emulsification by Bacterial Isolates Isolated from Local Soils | ||
| Engineering and Technology Journal | ||
| Article 20, Volume 32, 6B, June 2014, Pages 80-85 PDF (143.23 K) | ||
| DOI: 10.30684/etj.32.6B.20 | ||
| Authors | ||
| Salah H. Kalaf; Eman H. Gata; Amil A. Halob; Saad H. Khudhair | ||
| Abstract | ||
| The purpose of this research was to isolate local bacterial have the effectiveness of de-emulsification in liquid emulsion. Twenty local isolates of actinomycetes were isolated from soil contaminated with oil waste using mineral liquid medium at initial pH of 7 in the presence of oil waste (1%) as a source of carbon and incubated at 40 °C for 5 days . The primary screening of isolates was tested to de-emulsifying ability.Results indicate that the isolates ST3 , ST8 and ST15 were selected for theirhigh ability to de-emulsification. Secondary screening of selected isolates was estimated to deemulsification using the spore suspension . Results indicate that the ST8 isolate showed the highest de-emulsification activity, subsequently was identified and designated as Nocardia sp.. Studied the effects of both the spores number and period of exposure on the effectiveness of de-emulsification. The results showed that the 0.5 ml of spore suspension (107 spore/ml) gave the best effective way to de-emulsifying, while 30 min. the best period appropriate to de-emulsifying. | ||
| Keywords | ||
| emulsification; Actinomycetes; Nocardia sp | ||
|
Statistics Article View: 163 PDF Download: 54 |
||