Study on changes some metabolites composition of ovarian follicular fluid in relation to follicular size in local Buffaloes | ||
| journal of kerbala university | ||
| Article 1, Volume 10, Issue 4, December 2014, Pages 212-217 PDF (0 K) | ||
| Abstract | ||
| The aim of this study was to estimate the some metabolites composition of ovarian follicular fluid from different sized follicles and it's relationship with follicular size in local buffaloes. Ovaries were collected (80 ovary) from 40 non-pregnant female buffaloes which slaughtered at abattoirs of province of Babylon during the period from March to August 2014. The ovaries were transported to the laboratory within 2 hours post slaughter. Follicular fluid was aspirited from small (3-5mm), medium (6-10mm) and large (11-20mm) follicles and stored at -5ºC prior to assay. The follicular fluid samples were analyzed for glucose, total protein, triglycerides and total cholesterol, , and, using commercially available kits. The result showed that the mean concentration of cholesterol and glucose in follicular fluid of large follicles were significantly higher (P<0.05) than the follicular fluid in small follicles. While the mean concentration of triglycerides and total protein in ovarian follicular fluid of large follicles were significantly lower (P<0.05) than follicular fluid in small follicles. | ||
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