Spectrophotometric determination of Procaine Hydrochloride in Bulk and Pharmaceutical by Diazotization-Coupling Reaction | ||
| Iraqi National Journal Of Chemistry | ||
| Article 1, Volume 0, Issue 56, December 2014, Pages 368-380 | ||
| Authors | ||
| Kassim Hassan Kadim; Marwa Saad Mohammed | ||
| Abstract | ||
| Abstract Asimple,rapid,sensitive,selective,and accurate spectrophotometric method for the determination of microgram amounts of procaine Hydrochloride drug in pure form and in pharmaceutical preparations.The method is based on diazotization of primary amine group of (PRO-HCl) with sodium nitrite and hydrochloric acid followed by coupling with 1,3-phenylenediamine in aqueous mildly acidic medium to form a stable orang azo dye, showed absorption at 454 nm. Beer’s law is obeyed over concentration range of(0.015-7.000)µg.m L-1 with molar absorptivity of (6.906× 104 ) L.mol-1.cm-1 and Sandell’s sensitivity of (0.004).µ.cm-2.The method does not needed to temperature control or to solvent extraction. The optimum conditions for all colour development are described and the proposed method has been successfully applied for the determination of procaine Hydrochloride in bulk drug and pharmaceutical preparation vials with very good recoveries. The common excipients and additives did not interfere in this method. | ||
| Keywords | ||
| Procaine hydrochloride; Spectrophotometric; diazotization; Coupling; Phenylenediamine; pharmaceutical preparations | ||
|
Statistics Article View: 7 |
||