Influence of the season and ovarian follicular size on hormonal, metabolic and ionic changes of ovarian follicular fluid in native ewes. | ||
| journal of kerbala university | ||
| Article 1, Volume 10, Issue 4, December 2014, Pages 173-182 PDF (0 K) | ||
| Abstract | ||
| The study was conducted at laboratories of department of animal technical production ,Al-MusaibTechnicalCollege from 1/2/2011 to 1/2/2012 to investigate some biochemical composition of ovine follicular fluid in relation to season and follicle size. The female reproductive organs were collected from slaughter house of province of Karbala .A total of 240 samples of genital tract. The samples were transported to the laboratory within 2-3 hours post slaughter. The follicular fluid was aspirated from small (>4mm),medium (4-6mm)and large (≤ 7mm) follicle .The follicular fluid was stored at -4C° prior to assay. Follicular fluid sample were analyzed for estrogen hormone, follicle stimulating hormone ,glucose ,cholesterol ,total protein ,potassium and calcium .The results showed that the mean of estrogen hormone level at different seasons of the year were highly significant (p<0.01)with an increased follicular size(diameter) ,while the mean follicle stimulating hormone level decreased highly significant (p<0.01) with difference in follicular diameter.The mean of cholesterol level at different seasons of the year wasshowed highly significant increased (p<0.01) in follicular fluid with increased in follicular size. The mean of glucose level at different seasons of the year increased significantly (p<0.01) with increased follicular size , while the mean total protein level at different seasons of the year decreased in highly significant (p<0.01) with enlargement of follicular size.The mean of potassium level at different seasons of the year decreased highly significant (p<0.01) with increased follicular size, rather the mean of calcium level at different seasons of the year increased significantly (p<0.01) with increased follicular size. | ||
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