Effect of Explant type and Benzyladenine on Culture Initiation and Multiplication of Three Strawberry's Cultivars | ||
| Euphrates Journal of Agriculture Science | ||
| Article 1, Volume 6, Issue 4, December 2014, Pages 1-13 | ||
| Abstract | ||
| Abstract: The study was conducted to examine the effect of explant type and Benzyladenine (BA) on culture initiation and multiplication of three Strawberry's (Fragaria x ananassa Duch.) cultivars using plant tissue culture technique. Several experiments were conducted comprising study of two explant types (apical meristem and runner tip) of the three cultivars: Festival, Robican and Sweet charly response to surface sterilization and in vitro culture under various concentrations of the cytokinine BA. Results indicated that the lowest contamination percentage (5%) was achieved in Festival cv. which was significantly deferred from Robican and Sweet charly cvs. (15%), while apical meristem was lower contaminated (6.6%) than runner tip (16.6%). Murashige and Skooge (1962) (MS) medium with 0.5 mg/L BA gave the highiest percentage of in vitro response of runner tip for Festival cv. (90%) and the highiest number shoots/explant (6.2) while apical meristem for the same cultivar gave 5.1 shoots/explants. In multiplication stage, Festival cv. was the best and gave 12.43 shoots. BA at 1.0 mg/L was the best concentration and gave 16.7 shoots. The highest multiplication mean was achieved for culturing Robican cv. shoots on MS medium supplemented with 1.0 mg/L BA plus 0.1 mg/L IBA (17.6 shoots). Festival cv. record highest number of leaf/plantlet (24.02). The highest average of leaf number was achieved in Robecan (33.9 leaf). The above results is useful in the micropropagation protocol of Strawberry. | ||
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